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This presentation will provide an overview of Drug-Facilitated Sexual Assault. It will present the commonly used drugs, in addition to less common and recognizable drugs, their signs, symptoms, and prevalence. It will provide a review of the DFSA kit and specimen collection, best practices, and reference the latest 2017 SOFT guidelines for laboratory analysis in DFSA kits. Additionally, the presenters will provide an overview of the psychological impact of DFSA and present case studies.